New advances in stem cell technology, microfluidics, tissue engineering and bioprinting offer possibilities for development of tissue and organ models that are physiologically relevant providing new platforms for quicker and more reliable drug discovery, toxicology studies and preclinical safety assessment as well as in vitro disease modeling. These 3D models aim to provide an effective alternative to traditional 2D cell cultures and animal experimentation. Some of the approaches include advanced 3D organoids, organ-on-chips, microphysiological systems (MPS), and bioprinted anatomically accurate 3D structures. This session will cover current research, advancements, challenges, and prospects of 3D tissue and organ models and their biomedical applications.